Studies of collagen metabolism was carried out by investigating the level of procollagen type III(PNIIIP) in sera and the content of total collagen in liver tissue. Rate were devided by 3 groups young rats, adult rats and liver cirrhosis induced adult rats. For the induction of liver cirrhosis in rats CCI, was injected 2-times weekly into i.p, during 6 weeks or the rate were operated by bile duct ligation/scission. The circulated PNIIIP in sera was measured by radioimmunoassay and total collagen in liver tissue by colorimetry. For the observation of histological change in liver tissue was stained by Hematocylin and Eosin. The value of PNIIIP in young rats and cirrhosis induced adult rats was 5-6 times higher than that of normal adult rats (31.2+4.2ng/㎖, 3.0+1.3ng/㎖). The collagen content of liver tissue was 17.9+2.8㎍/g in adult rats. However the collagen value of cirrhotic liver in adult rats (CCL: 32.0+2.7㎍/g;, BDL/S: 35.2+6.5㎍/g, p$lt;0.005) and in young rat group(32.9+5.1㎍/g, p$lt;0.005) was significantly increased. The histological finding in GCI, group showed the massive inflammation, the formation of septum and hepatocyrtes regeneration. Moderate bile duct proliferation, weak inflammation and hepatocytes island were observed in the cirrhotic rats by operation of bile duct ligationr/scission.
It suggest that the high value of PNIIIP in sera and total collagen of liver tissue in young rats were influenced by growth hormone. The increased PNIIIP level in sera and the increased total collagen content in Liver tissue of cirrhosis induced adult rats show the stimulated synthesis of new fibrilla collagen f. g. PNIIIP and new formed fibrillar deposited broad and massive in periportal field.
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